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enzyme ddei  (New England Biolabs)


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    Structured Review

    New England Biolabs enzyme ddei
    Enzyme Ddei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ddei/Dde+I/pm42008180-93-26-28
    Average 96 stars, based on 856 article reviews
    enzyme ddei - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Single Cell:

    Article Title: A reproducible human brain tissue model to study physiological and disease-associated microglia phenotypes
    Article Snippet: .. Edited single-cell clones were analyzed by RFLP assay, using enzymes HpyCH4IV (NEB, Cat#R0619S) for APP Arctic, and DdeI (NEB, CatR0175S) for APP Iberian, followed by Sanger sequencing as described . ..

    Clone Assay:

    Article Title: A reproducible human brain tissue model to study physiological and disease-associated microglia phenotypes
    Article Snippet: .. Edited single-cell clones were analyzed by RFLP assay, using enzymes HpyCH4IV (NEB, Cat#R0619S) for APP Arctic, and DdeI (NEB, CatR0175S) for APP Iberian, followed by Sanger sequencing as described . ..

    RFLP Assay:

    Article Title: A reproducible human brain tissue model to study physiological and disease-associated microglia phenotypes
    Article Snippet: .. Edited single-cell clones were analyzed by RFLP assay, using enzymes HpyCH4IV (NEB, Cat#R0619S) for APP Arctic, and DdeI (NEB, CatR0175S) for APP Iberian, followed by Sanger sequencing as described . ..

    Sequencing:

    Article Title: A reproducible human brain tissue model to study physiological and disease-associated microglia phenotypes
    Article Snippet: .. Edited single-cell clones were analyzed by RFLP assay, using enzymes HpyCH4IV (NEB, Cat#R0619S) for APP Arctic, and DdeI (NEB, CatR0175S) for APP Iberian, followed by Sanger sequencing as described . ..

    Purification:

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer
    Article Snippet: .. Purified plasmid was digested with DdeI (NEB, Ipswich, MA) overnight at 37 °C, resulting in an ~3 kb DNA fragment. ..

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer.
    Article Snippet: .. Purified plasmid was digested with DdeI (NEB, Ipswich, MA) overnight at 37 °C, resulting in an ~3 kb DNA fragment. ..

    Plasmid Preparation:

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer
    Article Snippet: .. Purified plasmid was digested with DdeI (NEB, Ipswich, MA) overnight at 37 °C, resulting in an ~3 kb DNA fragment. ..

    Article Title: Single amino-acid differences define H2B variants and modify chromatin accessibility to induce EMT in breast cancer.
    Article Snippet: .. Purified plasmid was digested with DdeI (NEB, Ipswich, MA) overnight at 37 °C, resulting in an ~3 kb DNA fragment. ..

    Agarose Gel Electrophoresis:

    Article Title: The Ighmbp2-R604X mouse presents with the most severe SMARD1 clinical symptoms resulting in failure to thrive, respiratory and feeding deficits, aspiration and severe axon and muscle pathology.
    Article Snippet: .. Amplicons for the R604X assay were digested with DdeI (NEB) and separated on a 4% agarose gel to differentiate wild type from mutant alleles. ..

    Mutagenesis:

    Article Title: The Ighmbp2-R604X mouse presents with the most severe SMARD1 clinical symptoms resulting in failure to thrive, respiratory and feeding deficits, aspiration and severe axon and muscle pathology.
    Article Snippet: .. Amplicons for the R604X assay were digested with DdeI (NEB) and separated on a 4% agarose gel to differentiate wild type from mutant alleles. ..



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